全文获取类型
收费全文 | 1411篇 |
免费 | 91篇 |
国内免费 | 154篇 |
出版年
2023年 | 21篇 |
2022年 | 32篇 |
2021年 | 36篇 |
2020年 | 34篇 |
2019年 | 49篇 |
2018年 | 38篇 |
2017年 | 44篇 |
2016年 | 40篇 |
2015年 | 41篇 |
2014年 | 51篇 |
2013年 | 93篇 |
2012年 | 47篇 |
2011年 | 55篇 |
2010年 | 50篇 |
2009年 | 77篇 |
2008年 | 66篇 |
2007年 | 88篇 |
2006年 | 72篇 |
2005年 | 64篇 |
2004年 | 56篇 |
2003年 | 50篇 |
2002年 | 45篇 |
2001年 | 37篇 |
2000年 | 29篇 |
1999年 | 29篇 |
1998年 | 17篇 |
1997年 | 29篇 |
1996年 | 24篇 |
1995年 | 30篇 |
1994年 | 24篇 |
1993年 | 30篇 |
1992年 | 26篇 |
1991年 | 13篇 |
1990年 | 15篇 |
1989年 | 13篇 |
1988年 | 15篇 |
1987年 | 10篇 |
1986年 | 18篇 |
1985年 | 20篇 |
1984年 | 24篇 |
1983年 | 11篇 |
1982年 | 22篇 |
1981年 | 10篇 |
1980年 | 12篇 |
1979年 | 9篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 7篇 |
1973年 | 5篇 |
1971年 | 4篇 |
排序方式: 共有1656条查询结果,搜索用时 15 毫秒
1.
micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fused to the lacZ gene were constructed. Two distinct regions, which function differently, were identified as cis-acting regulatory elements, namely, one responsible for OmpR-dependent activation and the other for OmpR-independent repression of micF expression. The former contains the OmpR-binding site, which simultaneously regulates both the genes, micF and ompC, in response to the medium osmolarity. The latter may be involved in an unknown regulatory process of micF expression. 相似文献
2.
3.
4.
Moment-based criteria for determining bioequivalence 总被引:1,自引:0,他引:1
5.
Michael J. Wade Charles J. Goodnight 《Evolution; international journal of organic evolution》1998,52(6):1537-1553
We critically review the two major theories of adaptive evolution developed early in this century, Wright's shifting balance theory and Fisher's large population size theory, in light of novel findings from field observations, laboratory experiments, and theoretical research conducted over the past 15 years. Ecological studies of metapopulations have established that the processes of local extinction and colonization of demes are relatively common in natural populations of many species and theoretical population genetic models have shown that these ecological processes have genetic consequences within and among local demes. Within demes, random genetic drift converts nonadditive genetic variance into additive genetic variance, increasing, rather than limiting, the potential for adaptation to local environments. For this reason, the genetic differences that arise by drift among demes, can be augmented by local selection. The resulting adaptive differences in gene combinations potentially contribute to the genetic origin of new species. These and other recent findings were not discussed by either Wright or Fisher. For example, although Wright emphasized epistatic genetic variance, he did not discuss the conversion process. Similarly, Fisher did not discuss how the average additive effect of a gene varies among demes across a metapopulation whenever there is epistasis. We discuss the implications of such recent findings for the Wright-Fisher controversy and identify some critical open questions that require additional empirical and theoretical study. 相似文献
6.
Robert Blake II Elizabeth A. Shute James Waskovsky Arthur P. Harrison JR. 《Geomicrobiology journal》2013,30(3-4):173-192
Abstract Microorganisms capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. Phylogenetically distinct organisms were shown to express spectrally distinct redox‐active biomolecules during autotrophic growth on soluble iron. A new iron‐oxidizing eubacterium, designated as strain Funis, was investigated. Strain Funis was judged to be different from other known iron‐oxidizing bacteria on the bases of comparative lipid analyses, 16S rRNA sequence analyses, and cytochrome composition studies. When grown autotrophically on ferrous ions, Funis produced conspicuous levels of a novel acid‐stable, acid‐soluble yellow cytochrome with a distinctive absorbance peak at 579 nm in the reduced state. Stopped‐flow spectrophotometric kinetic studies were conducted on respiratory chain components isolated from cell‐free extracts of Thiobacillus ferrooxidans. Experimental results were consistent with a model where the primary oxidant of ferrous ions is a highly aggregated c‐type cytochrome that then reduces the periplasmic rusticyanin. The Fe(II)‐dependent, cytochrome c‐catalyzed reduction of the rusticyanin possessed three kinetic properties in common with corresponding intact cells that respire on iron: the same anion specificity, a similar dependence of the rate on the concentration of ferrous ions, and similar rates at saturating concentrations of ferrous ions 相似文献
7.
Many conclusions about mammalian ranging behaviour have been drawn based on minimum convex polygon (MCP) estimates of home
range size, although several studies have revealed its unpredictable nature compared to that of the kernel density estimator.
We investigated to what extent the choice of home range estimator affected the biological interpretation in comparative studies.
We found no discrepancy when the question asked covered a wide range of taxa, as real and very large differences in range
size were likely to have masked smaller differences due to the choice of home range estimator. However, when the question
asked concerned within-species characteristics, the choice of home range estimator explained as much of the variation in range
size as did the ecological variable in question. The implications for macro-ecological and intraspecific studies are discussed. 相似文献
8.
Eladio F. Galiano 《Plant Ecology》1985,63(3):121-127
The abundance of Cynodon dactylon was recorded in 50 plots presenting different geomorphological conditions and along a transect of 16 500 2×2 cm contiguous quadrats within a small basin 330 m in length, in the granite pediment of the Sierra de Guadarrama (Central Spain). Soil analyses were undertaken on samples from the 50 plots and the soil information matrix obtained was analysed using Principal Components Analysis and Discriminant Analysis. Results showed that in the Mediterranean pastures Cynodon dactylon was restricted to deep, well developed soils with relatively high values of cations, conductivity and organic matter, and could be used as an indicator of such soil conditions in these grasslands. The spatial pattern of the species was analysed using New Local Variances which revealed the existence of a small-scale pattern, ranging from 8 to 18 cm, present at all levels of the analysis. The size of small-scale patterns was positively correlated with species abundance and was interpreted as a relation between plant vigour and favourable soil conditions.I would like to thank Professor González Bernáldez for his interest and encouragement. 相似文献
9.
10.
Zonation of submerged macrophyte vegetation in Lake Kariba,Zimbabwe and its ecological interpretation 总被引:1,自引:0,他引:1
Cecil Machena 《Plant Ecology》1988,73(2):111-119
The submerged vegetation of Lake Kariba is described in relation to degree of slope (lake morphometry), depth and light transparency. The direct gradient analysis technique — canonical correspondence analysis and the TWINSPAN classification programs were used to analyse the data set. The western end of the lake with low transparency has a low species diversity (with Vallisneria aethiopica dominating). Species diversity increases with increased transparency in the other parts of the lake. The classification revealed monospecific communities for all species as well as mixed communities with Lagarosiphon as the associate species with the broadest distribution. The ordination revealed a first axis strongly related to depth and transparency gradients and the second axis related to slope. Vallisneria aethiopica has a growth form adapted to grow in shallow areas subjected to wave action. Potamogeton octandrus also growing in shallow areas is restricted to sheltered areas. Lagarosiphon ilicifolius precluded from the shallow exposed areas grows at medium depth but has a growth form adapted to tap light at the surface. Najas pectinata and Ceratophyllum demersum are adapted to grow in deep water.Abbreviations CCA
Canonical correspondence analysis 相似文献